Analysis of TCM injections using the Agilent 1290 Infinity LC system
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چکیده
An ultra high performance liquid chromatography (UHPLC) method using an Agilent 1290 Infinity LC system with an Agilent 1290 Infinity Diode Array Detector (DAD) was developed for the separation of Shenmai injections. Good separation of complex systems was achieved by connecting two columns: an Agilent ZORBAX Eclipse Plus C18 column (2.1 mm × 100 mm, 1.8 μm) and an Agilent ZORBAX SB C18 (2.1mm × 50 mm, 1.8 μm) column. These were chosen for their separation efficiency and good chromatogram peak shape. The results demonstrated that this analytical method was simple, sensitive and reliable for rapidly analyzing TCM injections, and is helpful in comprehensively evaluating the quality of the TCM injection. Authors Zhixiu Xu Agilent Technologies Shanghai Shanghai 200131 China Application Note Traditional Chinese Medicine
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